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KMID : 0900919860100010036
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Korean journal of Animal Reproduction
1986 Volume.10 No. 1 p.36 ~ p.41
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Sexing of Mouse Embryos by Chromosomal Analysis
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Han Yong-Mahn
Kim Jong-Bae
Park Hong-Yang
Lee Kyung-Kwang
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Abstract
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These experiments were carried out to obtain basic information necessary for sexing embryos by chromosomal analysis. To observe metaphase chromosomes, all embryos developed to blastocysts were cultured in Ho, pp. & Pitts’’ medium containing 0.001% Colcemid under the gas phase of 5% CO2 in air at 37^{circ}C for 2 hours. The sex chromosome of mouse embryos shown normal development after culture in medium containing H-Y antiserum (10%, v/v) and complement (20%, v/v) also was confimed by chromosomal analysis. The results obtained in these experiments were summarized as follows: 1. Among 89 mouse blastocysts, the number of embryos identified to have XX and XY chromosome was 22(25%) and 25(28%), respectively and 42(47%) embryos were not identified. 2. Of total 40 mouse balstocysts cultured in medium containing H-Y antiserum and complement, 23(58%) embryos which were able to be discriminated their sex chromosomes were identified to be XX bearing embryos. 3. Sex chromosomes of a number of embryos subjected to chromosomal analysis were not identified. This result may be due to absence or poor quality of metaphase spreads.
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KEYWORD
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